Western blot analysis of PNMAL1 in extracts of various cell lines. Samples were incubated with PNMAL1 Polyclonal antibody (Product # PA5-110187) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Western blot analysis of PNMAL1 in SKOV3, HepG2, mouse brain, mouse liver. Samples were incubated in polyclonal PNMAL1 antibody (Product # PA5-110187) using a dilution of 1:3000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.
Supplier Page from Thermo Fisher Scientific for PNMAL1 Antibody